Analysis of the Effects of Berberine on ABCG2 Drug Transport Activity

 

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Madeline J Chmell
Committee: Dana Emmert, Jeff Holmes, Langdon Martin

The ABCG2 gene is an ATP-dependent membrane transporter that has been shown to be over-expressed with an efflux in breast cancer drug treatment presence. Berberine, a natural medicine, has been shown to exhibit anti-carcinogenic properties through a mitochondrial pathway. Berberine, therefore, could be pertinent in the treatment of breast cancer, without the detrimental side affects associated with chemotherapy. A parent (MCF-7) cell line was cultured along with the ABCG2 expressing AdVp3000 cell line and after treatment with berberine at various concentrations, the viability was assessed through MTT assays and the transport of the drug was analyzed via fluorescence accumulation assays. Due to limits on time and resources, a DX1 cell line, expressing the P-Glycoprotein multidrug resistant protein was cultured, treated with berberine concentrations, and analyzed with fluorescence accumulation assays. The MTT assays yielded no significant data, however, a revised procedure for future study was established. The fluorescence assays, however, yielded significant results for the DX1 cell line. Though these results were significant, they were the opposite of the hypothesis. The DX1 cell line showed that berberine could actually be working in conjunction with the multidrug resistant protein, P-glycoprotein, rather than against it, while the MCF-7 control cell line did not show any trend amongst the controls, inhibitors, or berberine concentrations.